DNA Puppeteered Calipers for High-Throughput Structural Analysis of Complex DNA Nanostructures
Abstract
DNA-Puppeteered Calipers for for High-Throughput Structural Analysis of Complex DNA Nanostructures ABSTRACT DNA nanostructures have attracted great interest due to their potential in enabling various highvalue applications, including reconfigurable visible-light plasmonic and metamaterial devices, sensors based on nanopores or nanobarcodes, nanorobotic therapeutic-delivery vehicles, and tools for dissecting biophysical mechanism. However, DNA self-assembly is error-prone, therefore improved design methods are needed for this technology to reach its full potential. In particular, a need exists for a high-throughput method for analyzing structure and defects for DNA nanostructures. Here we propose DNA-Puppeteered Calipers, a high-throughput method for angstrom-resolution measurement of distances between, and identification of, randomly selected pairs of ssDNA handles displayed on the surface of complex 3D DNA nanostructures. In this method, the two handles loop out a segment of a long ssDNA (“the caliper”) whose ends, in turn, are suspended between a surface and a microbead pulled away by an externally applied force (e.g. acoustic, centrifugal, magnetic, optical). The resting height of each bead reflects the distance between the handles, and the positions of millions of beads can be recovered per hour at high resolution using Reflection Interference Microscopy (RICM). These single-particle measurements will reveal unprecedented levels of detail on static and dynamic heterogeneity of DNA nanostructures, and these datasets will prove invaluable for elucidating design rules for DNA nanostructures. ??
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 12, 2016
- Source ID
- N000141510073
Entities
People
- William M. Shih
Organizations
- Dana–Farber Cancer Institute
- Office of Naval Research
- United States Navy