Evaluation of non-lethal effects of N2 bubbles on marine mammal health and the potential role of immune activity in facilitating the development of dive related injury
Abstract
The major purpose of this project is to investigate the potential non-lethal effects of gas bubbles on immune function in marine mammals and address the hypothesis that a less reactive immune system serves a protective role against the development of DCS. Additionally we aim to investigate blow (exhaled breath condensate) sampling as a means to monitor complement activation and potential risk of DCS-like pathologies. For aims 1, 2 and 3, blood samples will be obtained from beluga whales (n=3) at the Mystic Aquarium, Mystic CT, as well as from stranded harbor seals, harp seals and grey seals (aims 1 and 2) which are admitted to the Animal Rescue Program at Mystic Aquarium between 2015 and 2016. Samples will also be collected from wild belugas belonging to the Bristol Bay and Point Lay, AK populations during live capture/release health assessments or subsistence hunts in 2015 and 2016 (target n=10 per season/per location). Initial processing will occur in the field and samples will be shipped back in liquid nitrogen for analysis of hormones and complement. For aim 4, blow samples will be obtained from belugas (n=3) at the Mystic Aquarium, as well as animals from the Bristol Bay and Point Lay, AK populations. Additional blood and blow samples are also being sought from partner institutions (e.g. Georgia Aquarium, Shedd Aquarium) which house belugas.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 12, 2016
- Source ID
- N000141512203
Entities
People
- Ph. D. Laura Thompson
Organizations
- Office of Naval Research
- Sea Research Foundation
- United States Navy