Effects of plasma products on immune cell function in vitro
Abstract
Innate and adaptive immune suppression, characterized by a reduced ability of immune cells to produce inflammatory cytokines (eg tumor necrosis factor (TNF)and interferon (IFN)~) when stimulated, are strongly associated with adverse outcomes in critically ill and injured patients. Plasma transfusion is associated with increased risks of nosocomial infection and maycontribute to immune suppression after injury. This is important because plasma transfusion, as an integral part of damage control resuscitation, is commonly administered to trauma patients, and increasingly used in the military setting. Given the frequency of plasma transfusion and the high burden of post-trauma infection in the combat setting, it is essential to understandpotentially immunosuppressive effects of plasma products.Limited reports suggest that plasma may directly suppress immune cell function, though none of these studies have evaluated different plasma products, which likely have different immunomodulatory potential. In particular, solvent-detergent treatment may removeimmunosuppressive effects of plasma by removing microparticles, bioactive lipids and other potential immunologic mediators. However, the immunologic effects of various plasma products, including fresh frozen plasma, thawed plasma, liquid plasma, and solvent-detergent (SD) treated plasma are unknown. The performer has developed unique in vitro transfusion models using functional immunophenotyping, resulting in multiple peer-reviewed publications demonstratingimmunosuppressive effects of red blood cell products. Their proposed studies take advantage of these models, and thus we are well suited to fill this important gap in knowledge. The proposed studies will test the hypotheses that 1.) Plasma preparations currently used for transfusion will suppress monocyte and lymphocyte function in vitro; and 2.) Different plasma products will result in different degrees of immune suppression, with SD plasma products being the least immunosuppressive.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 25, 2017
- Source ID
- N000141512565
Entities
People
- Jennifer Muszynski
Organizations
- Office of Naval Research
- The Research Institute at Nationwide Children's Hospital
- United States Navy