Equipment for Gas Channel Research
Abstract
Abstract Our laboratoryÕs studies of gas transport across cell membranes rely heavily on two facilitiesÑthe Protein Expression, Purification and Crystallization Core (PEPCC) and the Cellular Biophysics and Physiology Facility (CBPF). Support from two previous DURIP awards created a state-of-the-art PEPCC that provides recombinant proteins for molecular-biophysical studies of gas-channel proteins, including crystallization of these proteins. The funds requested for the current DURIP application would increase our yield of overexpressed membrane proteins for biophysical and crystallization studies, permit a critically needed expansion of our crystallization trials to lower temperatures, and enhance handling of larger crystals at lower temperatures. The single requested component for the PEPCC portion of this grant application is: a 14-liter fermentor that can operate in mammalian-cell culture mode or in fermentation mode. This device gives us control over temperature, pH, and dissolved O2 concentrations, allowing optimization of cell growth conditions. The CBPF supports work on gas transport across cell membranes. The requested funds will go towards the purchase of: (1) A Robotic Oocyte Injection system (2) a Robotic 2 Electrode Voltage Clamp system. (3) Devices that add functionality to our new state-of-the-art stoppedflow apparatus (purchased with ONR funds) for measuring gas fluxes across the membranes of red blood cells and lipid vesicles with reconstituted membrane proteins (4) a Nova Biomedical, Stat Prime Arterial Blood Gas Analyzer (5) a workstation for modeling gas transport across cell membranes. This computer system will greatly reduce the time required for intensive calculations. (6) an Oscilloscope for our EngineerÕs use when developing software which control various electrophysiological equipment and systems in our lab All of the requested items from DURIP would directly benefit the gas-channel projects supported by two grants from the Office of Naval Research.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- May 22, 2016
- Source ID
- N000141512920
Entities
People
- Walter Boron
Organizations
- Case Western Reserve University
- Office of Naval Research
- United States Navy