Identification of Physiological Mechanisms Mediating Blast-Induced Traumatic Brain Injury Using Tissue Engineered Biomimetics
Abstract
Elucidation of mechanisms mediating physiological effects which arise from blast-induced shockwaves and microcavitation remains a daunting task. A 3-university research consortium, each component with its unique experience and expertise, has been established to overcome the difficulties of determining potential brain injury mechanisms. At least two microcavitation systems that generate shockwaves and microbubbles have been developed and validated by the Xiao Laboratory at the Old Dominion University (Norfolk, VA). These systems have shown to produce peak pressures in the range of 10 MPa and generate microbubbles of 20 to 30 ?m in size. These characteristics of microcavitaition are suitable for in vitro studies of molecular mechanisms. The Cho Laboratory at the University of Texas at Arlington has been effectively using multi-modal imaging methods and microfabrication technology to monitor in real-time the cellular and subcellular responses induced by shockwaves and microcavitation in the tissue-engineered models of brain tissue and blood brain barrier (BBB). The Varner Laboratory at Purdue University (West Lafayette, IN) will exploit the recently developed automated computational models and validate the activation of cell surface receptors by the chemical and mechanical signals produced by microcavitation. Upon completion of the proposed work, we expect to establish a paradigm for mild traumatic brain injury mechanisms with the molecular details in response to explosive blasts, including the altered structure and function of the BBB. Successful outcome of the 3-university research consortium requires seamless integration of research efforts. As the PI of the proposal, Dr. Cho will utilize email communication, videoconference, visits to laboratories, and annual workshops and progress meetings among all the investigators, postdoctoral fellows, and graduate students to ensure the success of the proposed interdisciplinary and multi-institutional research endeavor.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 10, 2016
- Source ID
- N000141612140
Entities
People
- Michael H Cho
Organizations
- Office of Naval Research
- United States Navy
- University of Texas at Arlington