THIS GRANT IS A CONTINUATION OF N00014-12-1-0983 Tissue Actuators for Biorobotics and Biosensing Applications
Abstract
Short Work Statement: The proposed effort addresses the fabrication of functional, vascularized cardiac tissue for biorobotic and biosensing applications using a cell-laden hydrogel approach with engineered microvasculature. Specifically, hydrogel encapsulated primitive rat embryonic stem cells will be simultaneously differentiated into cardiac and endothelial lineages using the microenvironmental cues Wnt11 (a cardiognesis signaling molecule) and Wnt5a (an endothelial differentiation signaling molecule) that are spatially patterned. Cell-laden hydrogels offer several advantages over cell seeded scaffolds for tissue engineering including accommodating cells in 3-dimensional architectures, supplying a fully hydrated environment similar to the native environment, enabling co-culture of more than one cell type with control over their spatial organization, enabling higher yields of cell densities, and providing more uniform cell distribution. Current approaches to tissue engineering are not adequate for fabricating functional tissues with a defined architecture that comprise more than one cell type starting with embryonic stem cells. Thus, if successful, this effort will add novel tools for the fabrication of next generation biomimetic tissue structures.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 03, 2016
- Source ID
- N000141612207
Entities
People
- Ali Khademhosseini
Organizations
- Office of Naval Research
- United States Navy