Near infrared (NIR) effect on exosome-mediated inflammation in a cellular model of decompression sickness (DCS)
Abstract
Physically compromised divers have significant impact on the safety and performance of the Navy. Protecting divers against inflammatory effects requires a multidisciplinary approach for intervention at the cellular level by developing a novel therapeutic system to mitigate the immune response to hyperoxic and compressed gas conditions. This research will explore near infrared (NIR) therapy against exosome-based oxidative stress to reduce decompression sickness. This basic cellular scientific investigation will provide much needed advancement in the field of pulmonary critical care undersea medicine.The project aims to demonstrate whether a beneficial effect of NIR exists that prevents oxidative stress damage to pulmonary epithelial and endothelial cells undergoing biophysical stress of hypoxia and hyperbaric pressures similar to the microenvironment experienced by divers undergoing decompression sickness (DCS). It is hypothesized that treatment under controlled conditions using NIR will reduce the number of exosomes and alter their content thus reducing the damaging effect of hypoxia and hyperbaric stress on the pulmonary system. The PI will conduct in vitro studies with and without NIR exposure, collect exosome samples and conduct proteomic analysis for high resolution detection and responsive physiology. To test the effect of NIR, they will apply temporal offset of NIR using in vitro cell models. The results will be combined with in vitro studies conducted under hypoxic conditions on pulmonary epithelial cells and pulmonary endothelial cells to develop a biophysical dynamic model with kinetic parameters to define the dynamics and validate the effect of NIR to prevent DCS.The objective is to evaluate the protective effect of NIR and to adjust the NIR dose to be optimal under in vitro studies previously conducted utilizing an Integrated NIR test platform with hyperbaric and hypoxic chamber attachment. The aim of this research is to achieve a noninvasive treatment strategy to protect and increase the endurance of Navy diver against DCS. This proposal has one main theme: To identify the NIR-responsive molecular and biochemical mechanisms underlying the early response to hypoxia and hyperbaric conditions that lead to benefit in humans.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 12, 2016
- Source ID
- N000141612411
Entities
People
- Rosalinda Sepulveda
Organizations
- Office of Naval Research
- President and Fellows of Harvard College
- United States Navy