Seahorse metabolic assessment of distressed cells
Abstract
This grant is provided for purchase of cellular metabolic function analysis equipment that will fully establish a facility for assessing the linkage between alterations in cellular bioenergetic capacity and cellular mechanics. The investigator~s lab already includes i) atomic force microscopy (AFM) capabilities with total internal reflectance (TIRF) microscopy and ii) quartz crystal microbalance with dissipation (QCM-D) capabilities for assessment of cell mechanics as well as iii) fluorescence microscopy and image analysis tools for assessment of mitochondrial motility. The addition of the proposed Seahorse XFe24 instrumentation for the rapid, facile and simultaneous determination of mitochondrial respiration (oxidative phosphorylation; OXPHOS) and glycolysis in the form of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) is essential to advancing the Navy research goals and for enhancing the lab~s scientific education infrastructure. The equipment will consist of the Seahorse Bioscience XFe24 Analyzer that profiles cellular metabolic functions using label-free, solid-state disposable optical sensors. Coupled with the state of the art AFM-TIRF, QCM-D and epifluorescence microscopy instruments, the addition of the XFe24 Analyzer will provide the investigator with a suite of imaging and analytical tools which will profoundly enhance the pursuit of pioneering research and provide state-of-the-art equipment for training a new generation of investigators in understanding the interrelationships between cellular metabolic function, bioenergetic capacity and cellular mechanical states in health and disease. Understanding these interrelationships is particularly important in preventing or treating the array of pathological events that occur at the molecular and cellular level when cells are distressed as a result of ~toxic~ exposures, including extremes of thermal, chemical and environmental conditions against which warfighter personnel need protection. In addition, this metabolic analysis equipment, when coupled with the lab~s cell mechanics capabilities, can also be used to advance fundamental understanding of other health problems affecting military personnel and the population at large, including cancer, obesity, diabetes, metabolic disorders, immunology, cardiovascular function, neurodegeneration, and aging.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Sep 23, 2016
- Source ID
- N000141612742
Entities
People
- David M Eckmann
Organizations
- Office of Naval Research
- United States Navy
- University of Pennsylvania