Genotyping completion of samples in the Marine Recruit Archive
Abstract
In a previous ONR-funded project using Marine Recruit saliva samples, the PI identified genetic factors associated with Noise Induced Hearing Loss (NIHL) susceptibility: a single nucleotide polymorpohism (SNP) located within the Nucleolin gene with potential to be used as a biomarker or as new therapeutic target against NIHL and another 58 SNPs associated with NIHL located within 17 genes.The objective of this new project is to achieve completion of the genotyping of the Marine Recruits Saliva Archive to validate previous results and to investigate Copy Number Variants (CNV) and polymorphism of epigenetic DNA methylations associated with NIHL susceptibility. The PI will sample over 2 million SNPs from DNA samples using an Illumina Omnichip 2.5-8 chip and over 450,000 methylation sites with the Illumina Methylation 450k array platform.The results of this project would: 1) identify those SNPs as validated genetic predictors of NIHL susceptibility and 2) identify CNVs and methylation changes associated with NIHL susceptibility as new avenues of discovery. The long term objective is to correlate the mapped DNA to identified hearing loss features to establish a predictive model for this Marine population. This work has a high probability of direct translation into human use for prediction of sensitivity to noise as well as further investigate the efficacy of orally administered NAC since the NAC arm of the archive would also be investigated. The transitional nature of this research would give much needed information on NIHL susceptibility in a population of young healthy males.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Sep 30, 2016
- Source ID
- N000141612866
Entities
People
- Yohann Grondin
Organizations
- Office of Naval Research
- President and Fellows of Harvard College
- United States Navy