Three Dimensional Printed Scaffolds for Understanding Effect of Cavitation on Blood Brain Barrier
Abstract
Three Dimensional Printed Scaffolds for Understanding Effect of Cavitation on Blood Brain BarrierThe blood-brain barrier (BBB) com"prises specialized endothelial cells and separates blood from brain tissue. The BBB serves as a functional, metabolic, and immunolog"ical barrier that regulates passive and active transport. It protects the brain from harmful blood compounds and provides homeostasis for optimal neuronal function. Studying BBB function and dysfunction is important for biomedical research and traumatic brain injury (TBI).Organs-on-chips are a new class of microengineered models that build on the advantages of current in vivo and in vitro models. Using microfluidic in vitro BBB models provides a more physiologically relevant BBB model due to its more representative dimen"sions andgeometries, and because it allows exposure of the endothelium to physiological fluid flow. BBBs-on-chips are excellent mod""els as not only can the expression of individual markers that provide information on an organ level function be studied, but the per""meability of the cell barrier can also be immediately examined. Because of this, BBBs-on-chips hold promise for more accurate measur"ements of BBB functions by incorporating sensors and real-time readouts after TBI.The performer proposes to design soft three-dimensional printed and fibrous scaffolds as more physiologically relevant models to study the function of BBB. We will then characterize" the BBB model. The aspects that need to be taken into consideration when designing and testing BBB models are: permeability, shear"" stress, and transendothelial electricalresistance (TEER). The proposed research program expands our knowledge of materials systems"" and realtime characterization, and enables better understanding of cavitation effect in flexiblesystems.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 09, 2017
- Source ID
- N000141712620
Entities
People
- Nicole Hashemi
Organizations
- Iowa State University
- Office of Naval Research
- United States Navy