Mass Cytometry Platform for Integrated NIHL Research
Abstract
A Mass Cytometer will enable the long string of scientific breakthroughs we have achieved in Noise Induced Hearing Loss (NIHL) resea"rch, especially in the area of Genetic Susceptibility and newly discovered pathways, where we are lead performer in NIHL research in"" precision medicine. The platform establishes new research capabilities, accelerates basic investigations in hearing loss research o""f interest to the Navy, Marine Corps and the DoD. This technology increases experimental throughput by at least an order of magnitud""e for single-cell proteomics, phenotypes, signaling pathways. For example, presently we detect nucleolin, a potential biomarker of N""IHL susceptibility, known at to express least 9 variants, yet we can only detect the presence or absence of the dysregulated protein"". Of importance, this instrumentation will seamlessly integrate with our existing analytical systems enabling new investigations int""o NIHL prevention in our experimental systems. The equipment portals to existing laboratory procedures, integrates to cellular analy"sis of NIR platform in which metabolomics and limited proteomic analysis are currently performed. We have achieved numerous breakthroughs of high impact of interest to the Navy: 1) We have more than doubled the number of reported Genetic SNPs (single nucleotide po"lymorphisms) in humans associated with noise induced hearing loss, 2) discovered new biomolecular pathways predisposing humans to TT""S, 3) experimentally verified cochlear mechanosensory cells undergo apoptotic programmed cell death when placed under oxidative stre""ss conditions when dysregulated nucleolin is present, 4) suppression of inflammation response in cochlear cells using NIR and 5) rep""orted antioxidants administered via gastric route is not sufficient portal for administration, except under extreme conditions. Sign"ificant advances this new platform offers for advanced cell characterization in which flow cytometry and time-offlight mass spectrom"etry are combined, therefore cells labeled with metal isotope reporters gains the ability to measure more than 40 markers per cell.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Sep 29, 2017
- Source ID
- N000141712823
Entities
People
- Rick Rogers
Organizations
- Office of Naval Research
- President and Fellows of Harvard College
- United States Navy