Special Equipment Grant- Microfluidizer
Abstract
In this special equipment grant, we are requesting total funds of $ 39,725 for 1 year to purchase a Microfluidics Device (110Y Laboratory Microfluidizer Processor with High Pressure Capability). This instrument will allow us to disrupt yeast cells that are expressing gas-channel proteins, and thus enable the subsequent purification of the proteins for either crystallization or biochemical(e.g., reconstitution by Prof. Malmstadt) studies. The requested Microfluidizer will thus support our team~s efforts to understand the mechanisms of gas transport through channel proteins. The results yielded from the successful completion of our project will be a quantum leap for the fields of gas channels, itself a major paradigm shift in biology. Our work will totally reorganize our thinking of how gases cross membranes, and link molecular mechanism to whole-animal performance. These studies are highly relevant for the DoD missions (e.g., decompression illness, gas toxicity, acute mountain sickness, exercise performance, wound healing), and to the general public.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jul 10, 2018
- Source ID
- N000141812229
Entities
People
- Walter Boron
Organizations
- Case Western Reserve University
- Office of Naval Research
- United States Navy