Electrodermal activity during hyberbaric oxygen exposure: rat study
Abstract
The proposed project addresses Aim 1 of a collaborative study led by U. Connecticut with Duke U. and U. South Florida (this lab). Aim 1 will be done at USF in the laboratory of co-I Dr. J. B. Dean using unanesthetized, instrumented adult male Sprague-Dawley rats. The goal of this aim is to evaluate the utility of measuring electrodermal activity (EDA) across the skin as a physiological marker of an impending grand mal tonic-clonic seizure, which the hallmark symptom of central nervous system oxygen toxicity (CNS-OT) while breathing hyperbaric oxygen (HBO2). We hypothesize that increased EDA can be detected prior to onset of seizures while breathing HBO2 and thus used to predict an impending episode of CNS-OT. Increased EDA has been observed prior to onset of epileptic seizures at room pressure in rodents. To accomplish this aim, rats are anesthetized and implanted with a radio telemetric module for measuring various physiological parameters. At least one week later, under anesthesia, two EDA electrodes are attached to the rodent~s back using either surface electrode attachment or subdermal attachment. 24 hours later, the animal is placed in the animal chamber inside the hyperbaric chamber andthe two EDA wire leads are connected to the animal. The EDA lead wires are restrained by a tether harness. The animal is dived to 5 ATA HBO2 until onset of first and second bouts of seizures. The importance of this study is to determine if electrodermal activity, which can be easily monitored in animals and humans, is a useful physiological marker of an impending CNS-OT ~hit~; that is, does EDAincrease prior to seizure onset, and can it be used as signal for taking corrective action by lowering inspired oxygen and thus averting CNS-OT seizures.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- May 23, 2019
- Source ID
- N000141912314
Entities
People
- Jay B. Dean
Organizations
- Office of Naval Research
- United States Navy
- University of South Florida