Linking the microbiome to brown adipose tissue to enhance warfighter health
Abstract
Linking the microbiome to brown adipose tissue to enhance warfighter healthAn Office of Naval Research priority is to understand the functional link between the gut microbiome and BAT. This priority hopes to enable warfighters to better tolerate cold, and to provide a source of energy for long missions where eating/drinking is not feasible. We aim to uncover the optimal conditions for establishing a specific gut microbiome that promotes BAT development using the disruption of the regulator for G protein signaling 14 (RGS14-KO) mouse model as a target. The concept is that if we know the different microbes in RGS-14 KO mice compared to wild type (WT) and what those microbes eat/produce, we will have a pathway to emulate RGS-14KO conditions in warfighter~s digestive tracts to improve performance. The ultimate goal is to design a gut microbiome-based therapeutic approach to promote BAT development.Major Hypotheses:H1: RGS14KO mice have a unique gut microbiome compared to WT that plays a role in promoting BAT development and a healthy metabolic phenotype.H2: RGS14KO mice have unique microbial metabolites in their gut compared to WT that promote specific microbes that mediate the BAT development.H0: RGS14KO mice are indistinguishable from WT mice and the microbiome does not play a major role in BAT development.Project Objective:Specific Aim 1: Determine the microbiome in the RGS14 mouse by rRNA operon profiling, metabolomics, and targeted uptake/production of specific metabolites by the gut bacteria using stable isotope probing.Specific Aim 2: Verify the physiological effects of the gut microbiome on a WT mouse host using fecal transplant to directly compare how specific microbes influences the development of BAT.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 15, 2019
- Source ID
- N000141912443
Entities
People
- Sara L Campbell
Organizations
- Office of Naval Research
- Rutgers University
- United States Navy