METABOLIC SYSTEM FOR IN VIVO RODENT TESTING
Abstract
From carbon containing compounds, electrons and protons are shuttled into the mitochondrial electron transport chain, creating an el ectrochemical gradient. At the end of the chain, oxygen undergoes four-electron reduction to produce water with four protons, a reac tion that is coupled to ATP synthesis. The majority of inspired oxygen is used for this purpose, thus measuring oxygen consumption r ates is useful for determining mitochondrial efficiency and capacity. A small portion of oxygen consumed, however, undergoes one-ele ctron reduction to form superoxide. Increasing the inspired partial pressure of oxygen in normo- and hyperbaric conditions increases the amount of oxygen in the circulation that is delivered to tissues. Elevated cellular oxygen can lead to increased superoxide pro duction, and if in excess of cellular antioxidant defense mechanisms will result in lipid, protein and DNA oxidation. Because the lu ng and central nervous system are sensitive to changes in the partial pressure of oxygen, research on the pathogenesis of oxygen tox icity has primarily focused on these systems. Our understanding of how breathing an increased partial pressure of oxygen influences skeletal muscle mitochondrial function and oxidant production, and work capacity remains limited. The use of a metabolic system int erfaced to a treadmill designed for mice and rats will allow for this research to be performed. This work will serve as the foundati on for testing compounds directed towards preserving skeletal muscle mitochondrial health in hyperbaric oxygen, essential for mitiga ting oxygen toxicity and optimizing diver performance.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Oct 22, 2021
- Source ID
- N000142112961
Entities
People
- Heath G Gasier
Organizations
- Duke University
- Office of Naval Research
- United States Navy