Phospholipid detection in Traumatic Brain Injury and Alzheimer s disease
Abstract
Regulation of phosphoinositides, especially the tightly regulated signaling lipid, phosphatidylinositol(4,5)-bisphophate [PI(4,5)P2] have been implicated in Alzheimer s disease (AD) and traumatic brain injury (TBI). We propose to study the temporal and spatial accumulation of PI(4,5)P2 in an optogeneitcly stimulated synthetic paradigm. We wil also determine the content of phosphoinositides inhuman AD and TBI brain tissues and biofluids. This work is currently funded by Department of Defense Peer Reviewed Alzheimer s Research Program Convergence Science Research Award, McIntire (PI), Title: Optogenetic Regulation of Phosphoinositide Metabolism in Susceptibility, Resistance, and Resiliency to Alzheimer s Disease-AssociatedDeficits and Pathology. To support these DOD funded efforts,we propose to acquire ThermoFisher Integrion Ion Chromatography with Eluent Generation Quote $54,223.96. This instrument is state of the art used for ion chromatography and separation of polyphosphorylated species of phosphoinositides. This unique methodology is necessary for detection of anionic lipid species which cannot easily be isolated and quantified using other methods such as mass spectrometry due to their isobaric characteristics. For example, phosphatidyl inositol with two phosphate groups on the inositol ring can occur as phosphatidylinositol 4,5-bisphosphate or phosphatidylinositol 3,5-bisphosphate. Each of these lipids has dramatically different cell signaling properties and metabolic profiles. Multiple groups have the need to separate and quantify the abundance of these lipids in multiple experimental models including cell lines, mouse brain and human brain biospecimens. Acquisition of this instrument will facilitate collaboration in our institute as well as provide a unique methodology which can be applied across fields providing feasibility for innovative research programs and grant applications that could not be accomplished without this instrumentation. Further this unique and highly specialized methodology and expertise in phosphoinositide metabolism is often sought by collaborators external to the institution leading to productive and innovative advances in the field. This instrument will augment our researchenvironment and support collaborative research and quantification of phosphoinositide lipid species which is not possible without the ThermoFisher Integrion Ion Chromatography with Eluent Generation.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 12, 2023
- Source ID
- N000142312052
Entities
People
- Laura Beth Mcintire
Organizations
- Office of Naval Research
- United States Navy
- Weill Cornell Medicine