Biochemical investigation of barnacle cyprid adhesive using confocal laser scanning microscopy and 2-photon microscopy.
Abstract
Biochemical investigation of barnacle cyprid adhesive using confocal laser scanning microscopy and 2-photon microscopy.:The PI Dr. Nick Aldred is requesting funds to travel to the US and visit Dr. Andrew Mount in Clemson University, South Carolina, and Drs. Kathryn Wahl and Gary Vora at NRL. They are planning a collaboration for the study of the composition of the adhesive used by barnacle larvae which are a critical stage in the colonization of Naval vessel surfaces by this fouling organism. Funds are requested for travel, per diem and accommodation during a trip planned for 2 to 3 weeks, for Dr. Aldred only. All research expenses will be covered by the laboratories. The mechanism and the physicochemical nature of the barnacle adhesive are not well understood and most studies have focused on the role of proteins. A previous collaboration between Clemson and Newcastle indicated an unexpected property of barnacle larval adhesive: multiple components are released sequentially and they remain separate when they contact the surface. The initial component contains lipids and probably acts as a water displacer or surface primer. Furthermore, Clemson University has identified the presence of chitin in the adhesive. Since chitin, lipids, and proteins are common in arthropod cuticles, the team hypothesizes that the adhesive components and apparatus are modified versions of those used in the production of the cuticle. To test this hypothesis they need to identify the storage and addition location of chitin which may be anywhere between the larval adhesive glands and the termini of the antennules where the adhesive is released. Dr. Aldred is an expert in microscopic imaging techniques most suited for this study, including laser scanning confocal microscopy, multiphoton microscopy, Bitplane IMARIS 3D visualization methods, and other complementary imaging methods such as TEM and SEM. The planned experiments that will be conducted during the visit are expected to facilitate a strong collaboration between the three labs, and provide new insight into the biochemistry and mechanism of adhesion of barnacles to surfaces. The study of chitin and lipids should complement the current emphasis on proteins in the study of barnacle adhesion. The advanced knowledge will be valuable for the design of materials that can mitigate biofouling by barnacles. I recommend funding this proposal.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Nov 23, 2016
- Source ID
- N629091612215
Entities
People
- Nick Aldred
Organizations
- Newcastle University
- Office of Naval Research
- United States Navy