CSP - Structural Mass Spectrometry Workshop
Abstract
Mass spectrometry (MS) is becoming increasingly popular in the field of structural biology for analyzing protein 3D- structures and for mapping protein-protein interactions. Various MS-based approaches have been developed over the years to provide insights into the structural properties of protein complexes (see Figure). These include approaches such as hydrogen exchange and radical footprinting, which expose solvent accessible surface areas and interaction interfaces, as well as methods such as native MS, ion mobility separation, and cross-linking MS, which yield information on the heterogeneity, topology and packing of protein assemblies. Our aim is to bring together experts across the different disciplines of structural mass spectrometry, in order to demonstrate the scientific findings that can be obtained by this arsenal of tools.Different levels of information can be determined by distinct structural mass spectrometry approaches. The diagram illustrates the various mass spectrometry- based methods that will be covered in the workshop, (left side, highlighted in blue) and the structural information they provide (right side, illuminated in green). The lines in the center of the diagram connect a specific experimental approach with the relevant structural information that can be gained from it.Our overall vision is to increase the awareness of modern structural MS approaches, and inspire researchers from many different disciplines to incorporate these approaches in their research. We hope that the workshop would form a platform for exposing the biological questions and type of research that can be promoted by structural MS methodologies and technologies. A high standard professional workshop on Structural MS was not yet organized in the committee, and we are humbly glad that the Weizmann Institute and its students are the pioneers in hosting such an event.The workshop will gather researchers from all over the world and expose them to the structural MS field, covering topics as native MS, crosslinking, hydrogen-exchange, ion mobility, footprinting, and computational approaches for data integration. Each session will focus on one method and demonstrate the results and findings it generates. Two international experts will deliver each session to ensure full coverage of the topic and complementation.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 04, 2017
- Source ID
- N629091712019
Entities
People
- Michal Sharon
Organizations
- Office of Naval Research
- United States Navy
- Weizmann Institute of Science