The Pathogenesis and Therapy of Chromophobe Renal Cell Carcinoma: A Single-Cell RNA Sequencing Approach

Abstract

Chromophobe renal cell carcinoma (RCC), the third most common subtype of RCC, represents ~5% of all RCC. The prognosis for patients with metastatic Chromophobe RCC (ChRCC) is estimated to be less favorable than for metastatic clear cell RCC. Single-cell RNA-Seq (scRNA-Seq) is a relatively new method for investigation of the RNA molecules made by each individual cell within a tumor, including not only the tumor cells but also immune cells and blood vessels. Single cell ATAC-Seq (scATAC-Seq) is used to investigate which regions of the DNA are most active. We will use these state-of-the-art techniques to study ChRCC. Our central hypothesis is that profiling these tumors on a single cell basis will lead to a better understanding of what is driving the growth of ChRCC and thereby lead to new, highly effective treatments.

Document Details

Document Type
DoD Grant Award
Publication Date
Nov 19, 2019
Source ID
W81XWH1910550

Entities

People

  • Elizabeth P Henske

Organizations

  • Brigham and Women's Hospital
  • United States Army

Tags

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biology
  • Molecular and genetic basis of cancer.
  • Oncology (Cancer Research).