Freeze Dried Platelet Extracellular Vesicles as a Hemostatic Adjunct to Resuscitation for Prolonged Field Care
Abstract
Research objectives and rationale behind proposed work: In this proposal, we will explore how a dried product derived from platelets called lyophilized platelet derived extracellular vesicles (LPEVs) can act to stop uncontrolled bleeding and organ failure in trauma patients. We will measure the effects of LPEVs in improving outcomes in trauma models. Problem to be addressed: Stopping bleeding in trauma patients can save lives. Blood products, which stop bleeding, are not always available especially in remote or austere settings. Platelets, which form clots, are one of the most limited blood components since they have a short shelf life. This proposal will aim to develop a dried platelet derived product (LPEVs), with a long shelf life, that can substitute for platelets and stop bleeding when blood products are not readily available. This proposal will investigate LPEVs in three ways: First, understanding how LPEVs can mediate clotting and stop blood vessel leak. Second, understanding how LPEVs affect rats subjected to trauma and hemorrhage. Third, testing the ability of LPEVs to stop traumatic brain injury (TBI)-induced brain swelling and cerebral bleeding. Applicability and impact of the research: This research will help develop a new logistically feasible product, LPEVs, that can be used to treat patients who are bleeding and also suffering from other conditions that involve inflammation and vascular compromise. The information gained from these studies can ultimately assist in developing treatment protocols designed to save lives in both military and civilian settings and decrease the long-term effects of injury. Benefit to Service members, Veterans, and their families: The short shelf life of liquid stored platelets (5 days) makes it almost impossible to provide platelets on the battlefield or to the medics and doctors responsible for immediate treatment of bleeding combat casualties on the battlefield. LPEVs are predicted to be stable for as long as 2 years, are easy to use, and could be made available at all levels of care. This product may benefit Service members who are acutely injured and in the long run these therapies may protect their organ function, which can often be compromised years later.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Mar 10, 2021
- Source ID
- W81XWH2010216
Entities
People
- Shibani Pati
Organizations
- United States Army
- University of California, San Francisco