Does Dystrophin Restoration Reverse Epigenetic and Transcriptional Pathogenic Features in Duchenne Muscular Dystrophy?

Abstract

Duchenne muscular dystrophy (DMD) is a devastating pediatric disease caused by mutations in the dystrophin gene, leading DMD boys to a wheelchair, followed by premature death within the second/third decade of their life. There is not yet a definitive cure of the disease; however, the recent entry into clinical trials of gene therapies approaches aimed at restoring dystrophin in DMD boys prompted an interest to evaluate the full spectrum of alterations caused by dystrophin deficiency and whether they can be completely or partly restored upon gene therapy. This proposal will address this urgent issue by investigating the relationship between dystrophin deficiency, the integrity of the tri-dimensional structure of the genome and the gene expression output of DMD muscles, and by determining whether these alterations could be reversed upon dystrophin replacement by the expression of micro-dystrophin – a strategy currently used in clinical trials with DMD boys.

Document Details

Document Type
DoD Grant Award
Publication Date
Dec 05, 2021
Source ID
W81XWH2110905

Entities

People

  • Pier Puri

Organizations

  • Sanford Burnham Prebys Medical Discovery Institute
  • United States Army

Tags

Fields of Study

  • Medicine

Readers

  • Immunology and Pathology
  • Molecular and genetic basis of cancer.

Technology Areas

  • Biotechnology