Nanofluidic Analysis of Protein Transport, Adsorption and Kinetics
Abstract
The unique capabilities inherent in nanofluidic systems hold great promise for the next breakthrough in biomolecular analysis and separation. Our proposal involves using our knowledge and experience in nanofluidics to answer fundamental questions about biomolecular transport, adsorption and kinetic interactions in confined channels, towards the development of breakthrough bioanalytical tools. The major goals of this project are 1) designing and fabricating nanofluidic channels with which to investigate fundamental transport, absorption and kinetic behavior, 2) develop unique bioanalytical and biosensing tools and 3) use our fundamental understanding at the nano and micro-scale to build unique create systems. In the last five years, we have achieved many of our major goals, we (1) designed and fabricated nanochannels for the development of unique systems to probe the behavior of biomolecules in confinement, (2) investigated various protein and peptide systems in nanochannels (PEG-dedrimers, degradation of proteins, polyglutamate), (3) applied novel nanofluidic-based preconcentration systems for concentration of biomolecules, (4) develop unique biosensor systems with AgDNA nanoclusters, (5) developed a novel technique to determine the kinetics of biomolecules in nanochannels with non-equilibrium capillary electrophoresis of equilibrium mixtures, (6) determined the separation efficacy of ions in nanochannels with both pressure priven and electrokinetic flow, (7) developed a robust system with which to evaluate numerous surface coatings on nanochannels for the development of nanofluidic bioanalytical systems, (8) developed a new sensing technique using acoustic spectroscopy, and (9) studied the effects of tangential flow filtration and channel deformation in micro and nanofluidic channels. These accomplishments will be summarized in this final report.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 25, 2021
- Source ID
- W911NF1210031
Entities
People
- Sumita Pennathur
Organizations
- Army Contracting Command
- United States Army
- University of California, Santa Barbara