Direct Analysis in Real Time Mass Spectrometry for Extraction-Free Chemical Profiling of Live Biological Tissues and Fluids
Abstract
This proposal requests funds to acquire a Direct Analysis in Real Time mass spectrometer (DART MS) to support DoD-related research at the University of HawaiÔi. The mass spectrometer features a unique, open-air atmospheric pressure ionization source design that allows near-instantaneous chemical analysis of three dimensional objects (e.g., whole insects, leaves, microbial swabs) with minimal sample preparation. Samples are introduced into an open air space between the DART ion source and mass spectrometer inlet, producing a chemical fingerprint of small polar and nonpolar molecules. The chemical profile can be used to discern differences in metabolic activity, age, genetic background, and reproductive state of organisms. The DART MS system will be used to enhance ongoing research addressing how physiology is modulated by chemosensory perception. From worms to humans, the perception of certain smells and tastes has profound effects on lipid metabolism, lifespan, and reproduction. How and why this conserved biological process occurs is not well understood. Using the fruit fly Drosophila as a model, flies will be subjected to a panel of chemosensory cues related to survival and reproduction. DART MS will be used to rapidly assess changes in lipid and steroid hormone profiles following exposure. Establishing the relevant signal-types and conditions under which sensory perception alters physiology will be a major advance for identifying the underlying molecular pathways. Potentially, smells and tastes could be a simple, easily implemented way to manipulate metabolism and stress-resistance. The instrumentation will also facilitate other DoD-funded projects addressing marine biofouling, biodiversity assessment, and the conservation of endangered native species. Lastly, the system will support university courses on analytical instrumentation and forensic analysis in three different institutions on Oahu. Altogether, the DART MS system will provide a new analytical dimension not available in HawaiÔi and will have broad scientific and educational impact.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 07, 2019
- Source ID
- W911NF1610216
Entities
People
- Joanne Y Yew
Organizations
- Army Contracting Command
- United States Army
- University of Hawaiʻi System