An integrated laboratory system for the study of physiological effects and processing of hydrogen sulfide
Abstract
Hydrogen sulfide (H2S) has long been studied for its toxic properties. Recent research, however, indicates that H2S plays a role as a cellular signaling molecule, which has fueled research towards application of H2S as a therapeutic. Nonetheless, major questions remain about H2S metabolism, its regulation, and how physiological effects affect whole organismal functioning. In a project funded by the Army Research Office, we are using comparative analyses between closely related populations that differ in their ability to cope with disruptions of H2S homeostasis to elucidate H2SÕs physiological effects and processing. The project quantifies consequences of H2S exposure for organismal performance and genome-?--wide gene expression responses. Here, we are applying for funds to transform and streamline experimental and analytical procedures required for the study of how organisms are affected by and process H2S. The proposed investments will serve to improve and integrate methods developed by our laboratory. The creation of an integrated laboratory system for the study of physiological effects and processing of H2S will hinge on three components: establishing high-?--throughput phenotyping capacity with enhanced environmental control, broadening analytical capabilities, and establishing designated computational infrastructure. The proposed investments will allow for the seamless integration of the new laboratory system with existing infrastructure available to the project. The laboratory system will transform our approaches to study organismal responses to H2S from genes to fitness, enhance current DoD funded projects, as well as facilitate the collection of additional data and the implementation of complementary experiments. Basic knowledge of organismal H2S processing will be requisite its safe applications as a proposed therapeutic. This is of great value for the U.S. military, because H2S has the potential to profoundly affect practices in combat casualty care and the treatment of traumatic injuries.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 07, 2019
- Source ID
- W911NF1610225
Entities
People
- Michael Tobler
Organizations
- Army Contracting Command
- Kansas State University
- United States Army