Redox Responsive Dynamic DNA Nanomaterials; Research Area 8: Life Sciences
Abstract
The objective of the research effort is to develop DNA nanostructures that are responsive to the redox state of their environment. The research effort will encompass two specific aims: (1) Develop approaches to synthesize redox active nucleosides. These nucleosides will be incorporated into DNA to generate redox active DNA. The resulting DNA will be characterized with respect to stability and capacity to form stable duplexes in both the oxidized and reduced states. Developing a thorough understanding of these parameters will provide the basis for generating novel structural motifs whose shape can be controlled via changes in redox state. (2) Synthesize a series of novel DNA nanostructures whose shape can be dynamically controlled in a well-defined and reversible fashion by altering their redox state. Three redox responsive systems of increasing complexity and potential utility will be explored: (i) a DNA strand exchange system; (ii) a polymerization system whereby short oligonucleotides reversibly organize into long rods upon exposure to oxygen; and (iii) DNA nanocages designed to release their contents upon reduction. These nanostructures will provide insights into the potential utility and rules of using redox active bases to generate structurally dynamic molecular systems.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jan 12, 2017
- Source ID
- W911NF1610246
Entities
People
- Robert D. Kuchta
Organizations
- Army Contracting Command
- United States Army
- University of Colorado Boulder