High-speed Volumetric Two-Photon Microscope for the Study of Neural Circuits

Abstract

The Defense University Research Instrumentation Program (DURIP) is designed to improve the capabilities of U.S. Universities to conduct research and to educate scientists and engineers in selected technical areas of importance to national defense. The ARO core program is providing funding to support the acquisition of research equipment and instrumentation for this purpose. This proposal is for the purchase of components from Scientifica to build a two photon microscope configured specifically for calcium imaging. The proposed system includes a motorized stage, movable periscope, resonant scanning system, data acquisition workstation, pocket cells and the necessary collection of optic components to record on the green and red channels. The laser system, from spectra-physics is a Ti:Sapphire laser in the range 690-1040 nm, to provide the emission light to excite the sample. Finally the 3D projector from Hitachi will be used to provide patterned photostimulation in the sample. The P.I., Professor Jose Carmena, of the University of California Berkeley will use the equipment to augment and enhance research capabilities in the area of high-speed imaging ofneuronal responses from lOOs of neurons simultaneously to understand the function, development, role in behavior, and dysfunction of neural circuits.

Document Details

Document Type
DoD Grant Award
Publication Date
Jan 12, 2017
Source ID
W911NF1610453

Entities

People

  • Jose M Carmena

Organizations

  • Army Contracting Command
  • Office of the Secretary of Defense
  • University of California, Berkeley

Tags

Fields of Study

  • Physics

Readers

  • Neuroscience
  • Optical Physics and Photonics.
  • Research Science/Academic Research

Technology Areas

  • Directed Energy