Intracellular pathway explorations enabled by liquid based scanning microwave microscopy
Abstract
The communication between organelles in cells and the interaction of cells with electric fields inside cells is difficult to dissect in real time, since most probes are invasive and kill the cell. Recently, the Burke Lab and others have begun to explore the use of scanning microwave microscopy to further investigate the electrically active components of cells, such as mitochondria, and their role in intracellular pathways, such as cell death pathways. The objectives of this proposal are to further exploit recent technological advances in scanning microwave microscopy to measure: ¥ Quantitative capacitance measurement of single isolated organelles ¥ Capacitance of and between organelles in a single cell ¥ Fields and currents generated by organelles at audio to microwave frequencies ¥ Biological significance of the fields and currents and their role in cell death pathways ¥ Quantitative measurement of nanoantenna radiation patterns in a liquid environment The overall method of attack will be based on extensive preliminary studies by BurkeLab on mitochondria electrical activity and scanning microwave microscopy.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Sep 11, 2018
- Source ID
- W911NF1810076
Entities
People
- Peter J Burke
Organizations
- Army Contracting Command
- United States Army
- University of California, Irvine