Protein-Polymer Bioconjugate Structures Measured by Magnetic Resonance
Abstract
Protein-polymer conjugates provide an opportunity to stabilize proteins for challenging environments. The soldier will likely encounter such challenging environments in the field. These polymer stabilized proteins present capabilities that can be utilized for the detection of chemical or biological agents. Rational design of stabilized protein-polymer conjugates requires an understanding of the design rules that govern bioconjugate performance. Unfortunately, these design rules have eluded the field, with conflicting mechanisms of stabilization reported. The proposed research will develop nuclear magnetic resonance spectroscopy techniques for determining the interactions present between the synthetic macromolecule and protein within a protein-polymer bioconjugates. We will pair precision polymer synthesis with advanced nuclear magnetic resonance methods for protein structure determination to define the structure of protein-polymer conjugate at atomic resolution. When correlated with bioconjugate activity and stability, the technique will enable the extraction of design rules that can be used to produce improved protein-polymer conjugates, which can perform in harsh and challenging environments.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- May 28, 2019
- Source ID
- W911NF1910300
Entities
People
- Dominik Konkolewicz
Organizations
- Army Contracting Command
- Miami University
- United States Army