Prototyping DNA Origami For Mesoscale Electrochemical Biosensors
Abstract
The ability to rapidly detect active viral pathogens at the point of care or in the field is an essential component of warfighter protection. Currently, however, deployable rapid detection modalities cannot differentiate between whole (i.e., infectious) viruses and degraded viral components. Here we build on our previous success at synthesizing DNA origami capable of binding objects of virus-like dimensions and using them to construct electrochemical sensor devices. These devices have the potential to be developed into a point-of-care whole-virus detection system; however, before this can happen, fundamental device characteristics and assembly methods must be determined and optimized. The proposals aims are to i) Discover typical origami-electrode distance dependence on sensor output ii) Optimize origami functionality for maximal sensor output/gain and iii) Develop plug- and-play origami electrodes that are capable of being used by non-experts in the classroom, clinic and field; demonstrate these electrodesÕ robustness in an undergraduate laboratory. As well as informing our plan for a whole-virus sensor, data generated by this work should provide the DNA origami and biosensor communities design parameters for effective generalized mesoscale electrochemical biosensor design. This proposal utilizes best practices in training undergraduate researchers in biochemical nanotechnology.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 10, 2019
- Source ID
- W911NF1910326
Entities
People
- Philip S. Lukeman
Organizations
- Army Contracting Command
- United States Army