Designer Cellulosome for Consolidated Bioprocessing
Abstract
Lignocellulosic biomass could be a feedstock to produce valuable products (i.e. biofuel, biochemicals, etc.). However, enzymatic conversion of plant biomass into simple sugars is a major bottleneck due to enzyme and pre-treatment costs. Consolidated bioprocessing (CBP) might be an ideal approach for large scale cellulosic bio-production. Unfortunately, no microbe has been identified yet from nature to perform efficient CBP. To date, ÔcellulosomesÕ from anaerobic bacteria (e.g. Clostridium thermocellum) has been considered natureÕs finest cellulolytic machinery. Therefore, we propose to develop a cellulolytic yeast strain with superior enzyme complex for CBP, capable of degrading raw biomass and concurrently producing several valuable products. To engineer the cellulosome system into yeast, several components including a designer scaffoldin gene (SCipA) and anchoring protein gene (OlpB) will be designed by our lab and synthesized by Joint Genome Institute (JGI, USA). The SCipA contains 9 cohesins so we can precisely control the position of each cellulase enzymes. Similarly, the cellulase genes including endo-glucanases (EgIII), exo-glucanases (CBHII), beta-glucosidase (NpaBGS) and cellulase boosters (LPMO and CDH) will be fused with Dockerins of bacteria to facilitate their interaction with SCipA. Finally, those cellulosomal components will be engineered into the genome of Kluyveromyces marxianus, a yeast that can serve as an ideal industrial host. The newly engineered CBP yeast will be used to study the cellulolytic machinery with a superior enzyme complex, and to improve the soluble sugar release by the synergistic action of enzymes on the designer cellulosome
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 06, 2019
- Source ID
- W911NF1910434
Entities
People
- Wen-Hsiung Li
Organizations
- Academia Sinica
- Army Contracting Command
- United States Army