Enabling High-Throughput Analytical Workflows at Xavier University of Louisiana
Abstract
Project Summary/Abstract PI: Terry Watt Institution: Xavier University of Louisiana Title: Enabling High-Throughput Analytical Workflows at Xavier University of Louisiana We propose the acquisition of a system of analytical instruments for high-throughput workflows to complement and extend existing research and educational capabilities at Xavier University of Louisiana. Specifically, we propose to acquire a high performance liquid chromatography (HPLC) system coupled to a spotting robot for use with an existing matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometer (MALDI TOF/TOF MS). In addition, we are requesting a specialized workstation for analysis of proteomics data that will be obtained using the instrumentation. Xavier University of Louisiana is a primarily undergraduate institution and HBCU with many research-active, federally-funded faculty in the natural sciences. The acquisition of the proposed equipment will significantly enhance ongoing research and education projects within the Department of Chemistry, specifically: (1) Proteomics research: identification of substrates of lysine deacetylases. (2) Biochemistry research: high-throughput analysis of mixtures of reaction products from enzyme assays. (3) Course incorporation: inclusion of structured proteomics activities and data analysis in a biochemistry capstone course. (4) Research training: hands-on training of undergraduate students with analytical instruments in research laboratories and independent capstone projects. The instruments will also have applications for other research groups in Chemistry and Biology at Xavier University of Louisiana, in proteomics, protein biochemistry, materials chemistry, and sensor development. In each of these research and education projects, analysis of a mixture or potential mixture is necessary, with separation by a liquid chromatography (LC) step to generate many microliter-volume fractions distributed across an analysis plate for high-throughput analysis by mass spectrometry or fluorescence. In addition, the instrumentation will be available to a broader community, including faculty in the College of Pharmacy and in the New Orleans research community, including faculty at University of New Orleans, Tulane University, Loyola University New Orleans, and Louisiana State University Health Sciences Center - New Orleans.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Aug 19, 2019
- Source ID
- W911NF1910452
Entities
People
- Terry J Watt
Organizations
- Army Contracting Command
- Office of the Secretary of Defense
- Xavier University of Louisiana