Live imaging of dynamic protein behavior in cells
Abstract
Here we propose the acquisition of an inverted, wide-field fluorescence microscope that will advance the diverse DoD-related research interests of several labs within the Biology department at Tufts University. Specifically, we are interested in an imaging system with flexibility in imaging a range of cells and tissues, capabilities for working with live cells and time-lapse imaging, and novel computational clearing algorithms. Computational clearing detects and removes the out-of-focus background for each image, making the signal of interest directly accessible. At the same time, in the in-focus area, edges, and intensity of the specimen features remain. The members of the research team have interests in understanding complex regulatory signals that dictate cellular behavior. This ranges from understanding the behavior of individual transcription factors to understanding organogenesis and regeneration. Furthermore, our work relies on a range of model organisms, from single-celled yeast and slime molds to complex vertebrates (frogs and salamanders). The proposed instrumentation will enable the goals of this research as well as directly contribute to the training of dozens of undergraduate and graduate students involved in the work and across the department.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jul 09, 2020
- Source ID
- W911NF2010083
Entities
People
- Stephen M. Fuchs
Organizations
- Army Contracting Command
- Tufts University
- United States Army