Real Time Imaging System to Study Vital Sub-Cellular Organelles

Abstract

Funds are requested for the purchase of a confocal LSM900 Airyscan detector upgrade to our current inverted light microscope/AFM/scanning microwave microscopy (SMM) set up in order to study sub-cellular organelles in their vital dynamic state. This will be a major upgrade to our atomic force microscope (AFM), allowing for simultaneous photo-sensitive and high-resolution optical imaging to complement scanning microwave microscopy for real-time imaging of vital cells and physiologically active sub-cellular organelles (i.e mitochondria). This will enable long-term research on the structure and dynamics of sub-cellular organelles such as mitochondria and their electrophysiological role and will be the first integrated platform for such research purposes. Currently supported as well as planned research for this equipment include: 1) Scanning microwave microscopy (SMM) of vital mitochondria and single cells, 2) Studies of cancer and apoptosis, 3) Integration of olfactory proteins with nanoelectronics using single ion channel electrophysiology; 4) Study of extracellular vesicles in human serum; 5) More generally, biotronics, i.e. biology and the physical world. " It is understood that any developmental items and specially designed parts, components, accessories and attachments generated under this Defense Department agreement are being developed for both civil and military applications."

Document Details

Document Type
DoD Grant Award
Publication Date
Jul 09, 2020
Source ID
W911NF2010103

Entities

People

  • Peter J Burke

Organizations

  • Army Contracting Command
  • United States Army
  • University of California, Irvine

Tags

Readers

  • Nanoscale Plasmonic Nanotechnology
  • Neuroscience