Multi-Well Extracellular Flux Analyzer for Mitochondrial Stress Arrays
Abstract
This DURIP seeks to purchase a dedicated high throughput Seahorse XF live cell metabolic assay platform (XFe96) to perform oxygen consumption and extracellular acidification studies on cell line models. Measurement of these two endpoints allows mitochondrial respiration and glycolysis to be interrogated. This system will be utilized by researchers at the University of South Alabama Mitchell Cancer Institute and College of Medicine to examine the metabolic responses of cells exposed to environmental toxins and cells under stress in vitamin B deficient and proficient states. Warriors are exposed to harsh environmental conditions, toxic chemicals, strenuous activity and potentially limited nutrient sources in their operational environments. These conditions can promote injury to cells and DNA that impair cellular performance, induce mutations, and promote the development of disease. B vitamins from food or supplements provide critical cellular cofactors responsible for energy production, DNA/RNA synthesis, chromatin stability, epigenetic regulation, synthesis of neurochemicals, cell signaling, and remediating free radicals generated from stress. These important functions place cofactors derived from B vitamins at a critical nexus point where deficiency in B vitamins may hamper a warriorÕs performance and health with long-term consequences on performance, cognitive ability, and disease development or progression. In order to maintain warrior performance or even enhance warrior performance, a better understanding of the metabolic consequences of environmental exposure and B vitamin imbalance is needed. This research may identify areas for intervention that could mitigate acute exposure effects or enhance resistance to chronic environmental injuries. Additionally, it may provide crucial insight into using B vitamin supplementation strategies to improve long-term health and performance within the military population through personalized nutrition. The requested multi-well extracellular flux analyzer will contribute to the mission of the Army Research Office and the DoD by promoting research to determine mechanisms by which cells are injured from harsh environments and toxicants and potential strategies for mitigating or resisting injury under these conditions, while educating the next generation of investigators with cutting edge tools.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jul 09, 2020
- Source ID
- W911NF2010155
Entities
People
- Natalie Gassman
Organizations
- Army Contracting Command
- United States Army
- University of South Alabama