BIOLUMINESCENCE INTENSITY AS A NOVEL TOOL FOR O2 MEASUREMENT IN THE BRAIN (Neurophysiology of Cognition)
Abstract
Aerobic metabolism is of fundamental importance for all cells and tissues. Brain oxygen reserves are minimal and disruption of blood flow quickly causes irreversible injury. It has been speculated that deficits in oxygen delivery play a role in most, if not all, acute and chronic neurological diseases. Unfortunately, the current methodologies for measurements of tissue oxygen tension (PO2) are difficult, expensive, and indirect, likely explaining why only a few labs are currently studying brain oxygenation. We are here requesting funds to develop a simple, cost-effective, and precise method to measure changes in brain oxygenation with bioluminescence imaging. We have found the intensity of the bioluminescence signal of the oxygen-dependent enzymatic reaction of the protein, GeNL, and its substrate, Furimazine, can provide a direct readout of cortical PO2. We have expressed GeNL in cortical astrocytes in young wildtype mice. Pilot data collected in vivo in mouse cortex shows that BLI changes linearly with tissue oxygenation detected by the traditional Clark oxygen electrodes. We here propose to validate the enzymatic reaction of GeNL with Furimazine is oxygen-dependent and thereby an accurate measure of PO2. We also propose to investigate whether sleep deprivation affects oxygen tension during functional tasks in awake behaving mice. This Abstract is publically releasable.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 25, 2021
- Source ID
- W911NF2110006
Entities
People
- Maiken Nedergaard
Organizations
- Army Contracting Command
- United States Army
- University of Rochester