Unlocking the Cryo-Domain: Bio-Cryo-Tomography from Micro to Atomic Scale
Abstract
This proposal will support multiple ongoing research projects aligned with the ARO mission. These include (a) harnessing capabilities of microorganisms for applications from protection against nuclear hazards to providing rapid chemical sensing and production to the warfighter; (b) exploring the mechanobiology of chromatin as a means to control differentiation of stem cells used in tissue regeneration for conditions that affect the veteran and active duty population; (c) designing interfaces of bio-integrated devices for peripheral neuromodulation, stimulated healing, and monitoring of vital signs with broad applications for Army personnel; (d) understanding microstructure and non-linear materials properties in hydrated and freezing granular media for development of next generation defensive barriers and enhancing trafficability in remote environments; (e) characterizing electrochemical interfaces in energy materials, manufacturing, and corrosion; and (f) exploring links between microcalcification and bone metastasis in breast cancer. These research projects share the need for cryogenic imaging and tomography at multiple length scales. The overarching objectives of this proposal therefore are to (1) enable imaging of pristine, frozen-hydrated samples, both in 2D, and using tomographic Òslice-and-viewÓ approaches; (2) enable preparation of cryogenic samples for subsequent imaging by other cryo-techniques, including electron and X-ray microscopies; (3) enable transfer of samples at cryogenic temperatures and under high vacuum to other instruments. A fourth objective is to develop capabilities for analysis by atom probe tomography at cryogenic temperature (cryoAPT). Successful development of cryo-APT will enable a major leap: the ability to investigate the atomic scale structure and composition of a diverse set of samples, from interfaces to organelles and bacterial microcompartments all the way to natural and artificial synapses. In addition, we expect significant synergy for energy and low-dimensional quantum materials; nanomaterials for programmable self-assembly; and aqueous interfaces in the context of the water nexus. Taken together, cryogenic FIB-SEM imaging and cryoAPT will have a lasting impact on a broad range of topics relevant to ARO, ONR, and the mission of other DoD and federal agencies. Towards these objectives, this proposal seeks funds to equip an independently purchased Focused Ion Beam Scanning Electron Microscope (FIB-SEM) with load-locks, cryo-stage, cryo-manipulator, and vacuum/cryo shuttles and other necessary equipment and control software.
Document Details
- Document Type
- DoD Grant Award
- Publication Date
- Jun 25, 2021
- Source ID
- W911NF2110052
Entities
People
- Derk Joester
Organizations
- Army Contracting Command
- Northwestern University
- United States Army