Biodosimetry (USUHS/AFRRI)
Abstract
The Biodosimetry program capitalizes on findings under PE 0602787HP, Medical Technology, and from industry and academia to advance novel medical countermeasures into and through pre-clinical studies toward newly licensed products. Research findings are focused to advance the development and to produce the following: (1) To establish clinically certified processes to permit rapid assessment of radiation exposed specimens; (2) To access radiation exposure by developing and providing biological and biophysical dosimetry capabilities for acute, protracted, and prior radiation exposure; (3) To develop novel triage protocols for rapid assessment of radiation exposure; (4) To establish equipment triage automation to support the ability to manage mass-casualty radiation incidents around the globe. In addition to the primary achievement of research objectives, the program educates Federal employees as a benefit to the public they serve through Federal service, through support to civil authorities, and in non-Federal professional and academic collaborations. FY21 Accomplishments: (1)Sustain efforts to establish a quality and assurance control plan for measurements of dose by cytogenic chromosome aberration assay. (2)Continued the evaluation and validation of new radiation-responsive biomarkers in NHP and human models for biodosimetric diagnostic applications. (3)Established and extended the use of hematology biodosimetry algorithms for radiation-dose assessment using multiple cell-types biomarkers and animals. (4)Established, optimized and validated radiation-induced DNA damage based on a quantitive long amplicon real-time PCR assay. (5)Developed a quantitative inhibition PCR assay of mitochondrial DNA using long- and short amplicon PCR using human samples. (6)Developed a quantitative inhibition PCR assay of mitochondrial DNA using long- and short amplicon PCR using mouse samples. (7)Extended the utility of the premature chromosome condensation (PCC) assay investigating the effects of PBI v/s TBI exposures to assess the fraction of the body exposed to radiation. (8)Validated the HIRI algorithm concept using animals (i.e., baboons, canine, and mice) dose-response databases for an extended time window up to 10 to 14 days after exposure. (9)Established a RICA algorithm for assessment of H-ARS severity using NHP radiation dose-response model based on CBC cell types biomarkers using the METREPOL system. (10)In 2019/2021, 15 manuscripts were published.
Document Details
- Document Type
- Accomplishment
- Publication Date
- Oct 01, 2023
- Source ID
- efd62cd19d578febe81206001823cebf