Mechanistic Studies of Flavivirus Inhibition and Nanoparticle-Catalyzed Decontamination
Abstract
In this work, we characterized entry inhibitors and human monoclonal antibodies against dengue virus (DENV) and the developed disinfection systems made from titanium oxide nano-materials. For the DENV inhibition aims, we determined that the DN59 peptide functions as an inhibitor by the novel mechanism of causing the RNA genome to be released from the virus particle. We also investigated the mechanism of two other inhibitory peptides and showed that they interfered with virus:cell binding during entry. We also investigated the binding epitope and mechanism of action of broadly neutralizing human monoclonal antibodies against the dengue E protein and found that they recognized the highly conserved fusion loop and blocked fusion between DENV and cell membranes. We extended our decontamination work with catalysts made from titanium oxide nano-materials. We used voltage activation of our TiO2 materials to create a novel germicide, and we described a recyclable, self-cleaning sensor useful for detecting hydrophobic toxins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2016
- Accession Number
- AD1010519
Entities
People
- Jose C. Barreto
- Scott F. Michael
- Sharon Isern
Organizations
- Florida Gulf Coast University