Environmental DNA as a Tool for Inventory and Monitoring of Aquatic Vertebrates: Environmental DNA Sampling for Species Detection

Abstract

This guide is intended for managers and technicians that are first-time users of environmental DNA techniques. The focus is on species-specific detection; projects focused on whole ecosystem surveys (metabarcoding) may need to modify these guidelines. The field protocol details the approach we developed for remote wetlands and small to mid-order streams and is designed for portability and ease of use. In this approach, water samples are concentrated onto filter membranes using simple hand vacuum pumps, eliminating the need for heavy or expensive filtration equipment. Filters are preserved in ethanol in the field, with no need for cold storage during transport to the laboratory.

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Document Details

Document Type
Technical Report
Publication Date
Nov 15, 2017
Accession Number
AD1057716

Entities

People

  • Alexander K Fremier

Organizations

  • Washington State University

Tags

DTIC Thesaurus Topics

  • Biodegradable Plastics
  • Cellulose Esters
  • Cold Storage
  • Contracts
  • Department Of Defense
  • Detection
  • Environmental Security
  • Filters
  • Filtration
  • Inventory
  • Materials
  • Monitoring
  • Nitrocellulose
  • O Rings
  • Polymerase Chain Reaction
  • Sampling
  • Vacuum Pumps

Fields of Study

  • Environmental science

Readers

  • Computer Science.
  • Environmental Engineering
  • Wetland-Land-Environmental Management.