RAGE/Diaph1, Diabetes, and Kidney Disease: Mechanisms and Novel Therapeutic Strategies
Abstract
In Year One of the funded grant, we have substantial progress in three critical areas: First, we have established the breeding colonies for all four of the key mouse lines to test the roles of RAGE and DIAPH1 in podocytes and monocytes/macrophages in the pathogenesis of diabetes associated nephropathic changes in the kidney. As detailed in the full progress report, many of these animals (males and females) have already been rendered diabetic and are on time course. Second, we have optimized podocyte isolation procedures as indicated in the grant application. This is a key step which will enable us to probe mechanisms of RAGE and DIAPH1 biology in these cells. Third, Dr. Ramasamy identifies substantial progress in the development and validation of metabolomics and lipidomics assays here at NYU in order to understand detailed mechanisms of the role of these molecules in the diabetic kidney. Taken together, our work in Year 1 ensures that the feasibility of our proposed studies is assured and that through the coming year, our research team will begin to obtain important data to address the questions proposed in our Specific Aims.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2018
- Accession Number
- AD1063817
Entities
People
- Ann M. Schmidt
- Ravichandran Ramasamy
Organizations
- Grossman School of Medicine