Defining Alpha and Beta-Cell Crosstalk for the Treatment and Prevention of Diabetes
Abstract
During this funding period we have bred up mice for study, have begun to enroll mice into study and generate samples for analysis in both aims 1 and 2. The primary finding to report at this time is our work in sham and VSG-operated MIPCre+ and MIPCre- mice that do not contain the floxed GLP-1R allele. These additional control groups demonstrate that the MIPCre allele does not alter our glucoregulatory findings after VSG in mice. In addition, as a complementary approach, we have developed a new single cell RNA-sequencing method for the study of islets. We have used this approach to study islets treated with and without a GLP-1 receptor agonist from healthy human donors. These data reveal that GLP-1 receptor signaling in human islets increases alpha cell expression of the prohormone convertase needed for GLP-1 production, demonstrating that our model of beta cell GLP-1R function has translational relevance in humans.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2019
- Accession Number
- AD1094164
Entities
People
- Bethany P. Cummings
Organizations
- Cornell University