Multi-Modal Microscope for Biophysical Assessment of Optical Perturbation in the Neural System
Abstract
In this DURIP, we proposed to build a multi-modal non-linear optical microscope that can acquire cellular and molecular, biochemical and thermal information from the same location during optical stimulation from neural cells and tissue in vivo in a single combined framework. We have successfully built this instrument - Multimodal Advanced Nonlinear and Thermal Imaging System, or MANTIS, a microscope that is modular and integrated into a single platform. This instrument combines multi-photon fluorescence imaging, second harmonic generation (SHG) imaging, high resolution biochemical imaging with coherent anti-stokes Raman scattering (CARS), targeted vibrational mode imaging with stimulated scattering (SRS) and thermal imaging in such a way that we can reproducibly overlay the various images with < 1 micron precision. This instrument has enhanced our ability to perform the funded AFOSR research, and has become a unique resource within Vanderbilt as well as to the biophysics community at large to perform such integrated imaging. This combination of various non-linear microscopy modalities with thermal imaging has provided our engineering students with a unique opportunity to correlate information acquired with each domain and recognize the limitations and benefits of each technique.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 23, 2021
- Accession Number
- AD1144418
Entities
People
- Anita Mahadevan-Jansen
Organizations
- Vanderbilt University