RNA-directed Therapy for C9ORF72-Linked ALS

Abstract

In this reporting period, we have established and extensively validated a new mouse model of C9ALS that shows robust disease-relevant molecular and neuromuscular phenotypes. We have established a stem-cell based spinal and cortical differentiation paradigm that recapitulates development of repeat RNA foci and poly-dipeptide translation. We have also generated Cas13dbased adeno-associated viral vectors that robustly reduce these molecular phenotypes in cellular assays including stem-cell derived spinal and cortical organoids

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2020
Accession Number
AD1145069

Entities

People

  • Gene W. Yeo
  • Kathryn Morelli
  • Stefan Aigner

Organizations

  • University of California

Tags

DTIC Thesaurus Topics

  • Alzheimer Disease
  • Animal Structures
  • Biomedical Research
  • California
  • Cells
  • Chromosomes
  • Covid-19
  • Department Of Defense
  • Diseases And Disorders
  • Genetics
  • Health Services
  • Medical Personnel
  • Motor Neurons
  • Nervous System
  • Nervous System Diseases
  • Neurodegenerative Diseases
  • Neurons
  • Spinal Cord
  • Stem Cells

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology