Evaluation of a Future BSL-3 Capability: Aerosol Generation and Collection on Filters
Abstract
This study evaluated aerosol release into the environment as a result of aerosol generation and capture onto a test filter. This was conducted to simulate an operation in the biosafety level 3 (BSL-3) laboratory and determine whether any organisms were released into the environment. Air and surface samples were collected during blank (no aerosols) and hot (with aerosols) runs, and the numbers of organisms per sample were averaged. Statistical tests indicated that there was no statistically significant difference in the environmental contamination for the blank and hot runs for air and surface samples. The following conclusions were reached: (1) The Sono-Tek aerosol generator (Sono-Tek Corporation, Milton, NY) is easy to use, and the BSL-3 group preferred it over the inkjet aerosol generator. (2) Filters directly attached to the aerosol generator prevent aerosol release to the environment and are preferred by the U.S. Army Combat Capabilities Development Command Chemical Biological Center (Aberdeen Proving Ground, MD) safety office and the BSL-3 group. (3) It is important to ensure that the glovebox components are easy to decontaminate. (4) Noncorrosive equipment should be used in the BSC or glovebox so they can be sprayed with decontamination material to decontaminate all surfaces.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2021
- Accession Number
- AD1150761
Entities
People
- Daniel Mcgrady
- Jana Kesavan
- Jerold Bottiger
Organizations
- United States Army Soldier Systems Center