Investigation on the Tuneable Optical Properties of Reformatted Bacterial Cellulose

Abstract

The investigations suggest the type of drying process and incubation period can alter the optical and material properties of bacterial cellulose. Press dried films showed better optical transmission as compared to freeze dried films but lower reflectance. Material characteristics of press dried films differed from freeze dried films, nanofibers were compactly packed and displayed lower crystallinity.The DOE investigations suggest that only certain factors affect BC microparticles yield and uniformity, while other factors set to a constant value. Media flow rate, incubation type and bacteria ratio affect BC microparticle yield significantly. Optimum harvest of inclusion bodies is crucial to ensure high yield of reflect in protein. Purification of reflect in protein through FPLC is not as effective as the HPLC method as performed in previous literature. BC biofunctionalization using co-culture of E. coli secreting m6A-curli peptides was successful. Key Achievements reported: Obtained optimum film thickness for optical tunability; optical transmission was better when the films were press dried.

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Document Details

Document Type
Technical Report
Publication Date
Apr 17, 2023
Accession Number
AD1211787

Entities

People

  • Sierin Lim

Organizations

  • Nanyang Technological University

Tags

Communities of Interest

  • Advanced Electronics
  • Sensors

DTIC Thesaurus Topics

  • Bacteria
  • Cellular Structures
  • Chemistry
  • Confocal Microscopy
  • Culture Techniques
  • Diffraction
  • Electromagnetic Spectra
  • Experimental Design
  • Fibers
  • Films
  • Flow Rate
  • Freeze Drying
  • Light Scattering
  • Liquid Chromatography
  • Materials
  • Materials Science
  • Microparticles
  • Nanoparticles
  • Optical Properties
  • Particles
  • Production
  • Refractive Index
  • Scattering
  • Spectra
  • Thin Films

Readers

  • Mathematics or Statistics
  • Molecular and Cellular Biochemistry
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology