Development and Evaluation of a Two Chamber Model for the in Vitro Analysis of Bacterial Leakage Following Endodontic Therapy
Abstract
Introduction: This In vitro study evaluated a two-chamber model for determining the frequency of bacterial leakage in root canal systems. This study evaluated root canals obturated with BC bioceramic sealer and AH Plus resin epoxy sealer in conjunction with gutta percha, when challenged with human saliva. The aim of this study was to determine if the current laboratory-based methods for evaluating bacterial leakage are sufficient. Materials and Methods: A model consisting of two chambers, one containing human saliva and one containing brain heart infused broth was evaluated. Two separate evaluation studies were conducted with a total of 35 extracted, single rooted teeth, each containing a single canal system. The teeth were then monitored for bacterial leakage by the formation of turbidity in the BHI broth. Each incident of bacterial leakage as demonstrated by turbidity was recorded. Results: 100 percent of experimental teeth leaked. No statistical analysis was performed due to lack of data. Conclusion: Future research should investigate the effectiveness of cold plasma sterilization to bacterial leakage models and strive to improve the physical design of the bacterial leakage apparatus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2023
- Accession Number
- AD1222316
Entities
People
- Brittany E. Bartenstein
Organizations
- Uniformed Services University of the Health Sciences