Biological Synthesis of a Protein Analogue of Acetylcholinesterase: Monoclonal Anti-Idiotype Antibody Analogue of the Esteratic Site.

Abstract

During the second year of this contract, we have purified human erythrocyte acetylcholinesterase to >87% homogeneity by MAb affinity chromatography and size-exclusion HPLC and have obtained sequence data for >100 residues, derived from tryptic, chymotryptic, and V8 peptides. We have used these sequence data to synthesize oligonucleotide probes with which to screen two human cDNA libraries for the gene encoding human AChE. In addition, we have identified a large number of MAb that bind to acetylcholinesterase and we have begun to characterize several of these antibodies that are capable of inhibiting acetylcholinesterase. Keywords: Monoclonal antibodies.

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Document Details

Document Type
Technical Report
Publication Date
Jul 10, 1985
Accession Number
ADA193469

Entities

People

  • J. T. August

Organizations

  • Johns Hopkins University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Albumins
  • Amino Acids
  • Antibodies
  • Biomedical And Dental Materials
  • Blood
  • Blood Cells
  • Cells
  • Enzyme Inhibitors
  • Erythrocytes
  • Genetic Structures
  • Inhibition
  • Inhibitors
  • Molecules
  • Protein Sequence Analysis
  • Proteins
  • Sequence Analysis

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Neurotoxicology