Analytical Method Development and In Vivo and In Vitro Exposure Studies of Bis-(Trifluoromethyl) Disulfide

Abstract

A GC/EC analytical method was developed to quantitate bis- (trifluoromethyl)disulfide accurately in exhaled air of exposed animals. Using the headspace sampling technique qualitative determination of unbound compound in biological sample can be achieved. Partition ratio in plasma was much higher than in water (4.26 vs. 0.14). In vitro studies indicated that irreversible binding and interaction could occur when bis-(trifluoromethyl)-disulfide was present in the blood. The unbound portion disappeared at a faster rate from RBC than from plasma. Bis-(trifluoromethyl)disulfide might have penetrated the lung tissue and entered the blood stream, but we did not detect any unbound compound in blood from exposed animals. The results could be explained by irreversible binding and sequestering action by blood components. Keywords: Pesticides; Toxicity; Organohalogen; Gas chromatography; Electron capture; Binding; Partition ratio; In vivo; In vitro tests.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1989
Accession Number
ADA211983

Entities

People

  • John R. Smith
  • Ming L. Shih

Organizations

  • United States Army Medical Research Institute of Chemical Defense

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biological Sciences
  • Biomedical Research
  • Blood
  • Blood Cells
  • Calibration
  • Chemical Synthesis
  • Chemistry
  • Classification
  • Detectors
  • Electron Capture
  • Electrons
  • Military Research
  • Sampling
  • Security
  • Spectrometry
  • Standards

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Toxicology/Environmental Toxicology

Technology Areas

  • Microelectronics