Characterization of Biopolymer Surfaces Using Scanning Microscopies

Abstract

The objective of this contract was to establish the feasibility of studying proteins on surfaces by scanning tunneling microscopy (STM) and atomic force microscopy (ATM). Amino acids and proteins deposited on highly oriented pyrolytic graphite (HOPG) were viewed by STM (Appendices A-C). A preliminary experiment was conducted in the laboratory of Dr. Paul Hansma, University of California, Santa Barbara, which demonstrated the observation of immunoglobulin (IgG) on quartz and mica surfaces. An underwater real time observation of IgG adsorption on mica (Appendix D) suggests that the process is not homogeneous. We now have a functioning AFM in our lab - (thanks to the generosity and assistance of Paul Hansma and coworkers) - and protein imaging studies are in progress. Keywords: Protein adsorption; Immunoglobulin adsorption.

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Document Details

Document Type
Technical Report
Publication Date
Nov 15, 1989
Accession Number
ADA216572

Entities

People

  • J. D. Andrade
  • J. N. Lin
  • Lin Feng
  • W. Bascom

Organizations

  • University of Utah

Tags

Communities of Interest

  • Advanced Electronics

DTIC Thesaurus Topics

  • Albumins
  • Amino Acids
  • Aqueous Solutions
  • Biopolymers
  • Chemical Synthesis
  • Chemistry
  • Crystal Lattices
  • Crystal Structure
  • Crystals
  • High Resolution
  • Materials Science
  • Membrane Lipids
  • Microscopes
  • Piezoelectric Crystals
  • Proteins
  • Surface Chemistry
  • Three Dimensional

Readers

  • Academic Conference Management
  • Electrochemical Surface Science
  • Molecular and Cellular Biochemistry