Characterization by NMR and Fluorescence Spectroscopy of Differences in the Conformation of Non-Aged and Aged Organophosphoryl Conjugates of AChE

Abstract

The objective of this study was to detect and characterize by nuclear magnetic resonance (nmr) and optical spectroscopy and by X-ray crystallographic study structural differences between non-aged and aged organophosphotyl (OP) conjugates of acetylcholinesterase (AChE) and chymotrypsin (Cht) which might help to explain the unexpected resistance of the aged form to commonly used reactivators. During the period covered by this report we synthesized novel organosphosphate inhibitors of serine hydrolases, performed spectroscopic studies on the conformational stabilization of aged and non-aged OP-Cht and OP- AChE conjugates and investigated OP-Cht conjugates by 31 P-nmr spectroscopy. In addition, we prepared crystalline OP-Cht conjugates and determined their structure by X-ray crystallography.

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Document Details

Document Type
Technical Report
Publication Date
Mar 15, 1990
Accession Number
ADA238708

Entities

People

  • I. Silman
  • Y. Ashani

Organizations

  • Israel Institute for Biological Research

Tags

Communities of Interest

  • Biomedical
  • C4I

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Chemical Shifts
  • Chemical Synthesis
  • Chemistry
  • Crystal Structure
  • Crystallography
  • Displacement Reactions
  • Enzymes
  • Geometry
  • Magnetic Resonance
  • Organic Chemistry
  • Organophosphates
  • Spectra
  • Spectroscopy
  • Three Dimensional
  • X Rays
  • X-Ray Crystallography

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Neurotoxicology